Poclightbio: Do you know about chemiluminescence?

 In vitro diagnostics  (IVD), refers to products and services that obtain clinical diagnostic information by testing human samples (various body fluids, cells, tissue samples, etc.) outside the human body, and then judge diseases or body functions. In vitro diagnostics is known as the "doctor's eye" in the medical field, and is an important part of modern laboratory medicine, and its clinical application runs through the whole process of disease treatment such as disease prevention, preliminary diagnosis, treatment plan selection, and efficacy evaluation, providing doctors with a large amount of useful clinical diagnostic information, and increasingly becoming an important part of human disease diagnosis and treatment.

IVD classification:

According to the different detection principles and methods, in vitro diagnostics can be divided into biochemical diagnosis, immunodiagnosis, molecular diagnosis, microbial diagnosis, blood diagnosis, point-of-care testing (POCT), etc.

IVD-Immunodiagnostics Market:

The IVD market is relatively scattered in various tracks, and immunodiagnosis is a subdivision with a large proportion of the market size. After iterative technology updates, immunodiagnostics has surpassed biochemical diagnostics and rapidly grown into a market segment with a large market scale in vitro diagnostics, with diverse diagnostic technologies and wide application coverage.

Immunodiagnostic classification:

Immunodiagnostics are the use of specific binding between antibodies and antigens to detect specific proteins, pathogens, or other molecules. According to the detection principle, it can be divided into enzyme-linked immunoassay, fluorescence immunoassay, chemiluminescence immunoassay, etc.

Fundamentals of chemiluminescence:

In the presence of antigens and antibodies, the binding region of the antibody binds to the epitope of the antigen to form an antigen-antibody or immune complex. Estimating the levels of this immune complex by using labeled antibodies is the basis of CLIA. It involves the use of a stationary solid phase of the antigen or antibody to be detected. This results in the generation of light, the intensity of which is proportional to the number of labeled complexes present and indirectly aids in the quantification of the analytes to be examined. The intensity of light is measured in relative units of light (RLU).

Advantages of chemiluminescence detection:

01 High sensitivity

High sensitivity is a key advantage of chemiluminescent immunoassays, with sensitivities of up to 10-22 mol/L (RIA10-12 mol/L). Chemiluminescence immunoassay can detect substances that cannot be detected by radioimmunoassays and enzyme-linked immunoassays, which is of great significance for the early diagnosis of diseases.

02 Wide range of linear dynamics

There is a linear relationship between the luminous intensity and the concentration of the measured substance between 4~6 orders of magnitude. This is an advantage over the chromogenic enzyme immunoassay absorbance (OD) range of 2.0.

03 The optical signal lasts for a long time

The glow-based CLIA produces light signals that can last for hours or even a day, simplifying experiments.

04 The analysis method is simple and fast

The vast majority of analytical assays are one-step modes in which only one reagent (or combination of reagents) is added.

05 The results are stable and the error is small

The sample is directly luminous and does not require any light source irradiation, which eliminates the influence of various possible factors (light source stability, light scattering, light wave selector, etc.) on the analysis, making the analysis results sensitive, stable and reliable.

06 Good safety and long service life

The use of radioactive substances has been exempted, the hazards of which have not been found so far; The reagent is stable and can be stored for more than six months to more than one year.

07 The test items are complete

It covers hundreds of indicators such as gonadal index detection, thyroid function, tumor markers, metabolism, etc.

Poclight, as a manufacturer, it has independent research and development patents. And our products, including high quality chemiluminescence immunoassay analyzer for lab use, with features dry mini portable handheld clia analyzer, and small clia system for vet use, can bring you fast, accurate and easy tests. If you are interested in our China OEM Customized Laboratory Clinical Emergency Portable Poct Micro Chemiluminescence Immunoassay Analyzer, welcome to contact us, and we can provide you with  trusted supplier of lab and poct solutions.